« Back
Home /
Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14)

Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14)

Manufacturer	IchorBio
Category	Oncology Antibodies In Vivo Antibodies By Cell Type Carcinoma Hematology Lymphoma Myelofibrosis Western Blot Blocking
Type	Antibody
Specific against	Mouse
Clone	RMP1-14
Isotype	IgG2a Kappa
Applications	WB, Blocking
Amount	1mg
Host	Rat
Item no.	ICH1132-1MG
Targets	CD279
eClass 6.1	32160702
eClass 9.0	32160702
Available
Aggregation	Aggregation level <= 5%
Antigen Distribution	Subset of double negative thymocytes, activated T and B cells
Application Notes	Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	<= 1.0 EU/mg as determined by the LAL method
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Immunogen	Mouse PD-1 transfected BHK cells
Product Type	Low Endotoxin, anti-mouse
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Storage	anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is stable when stored at 2-8C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1mg

available

Delivery expected until 6/6/2024