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Anti-Human/Mouse BACE-1 Ectodomain - 500 µg

Anti-Human/Mouse BACE-1 Ectodomain - 500 µg

Item no.	LEIN-B596-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IHC
Clone	137612
Specific against	Human (Homo sapiens)
Host	Mouse
NCBI	23621
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, FC, IHC FFPE, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	BACE-1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	BACE-1 is an aspartic protease and an integral membrane protein. BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein β site in the brain to generate the amyloid β peptide. Because the amyloid β peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 -5.5), consistent with its proposed function in an acidic intracellular compartment.
Background	BACE-1 is an aspartic protease and an integral membrane protein. BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein β site in the brain to generate the amyloid β peptide. Because the amyloid β peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 -5.5), consistent with its proposed function in an acidic intracellular compartment.
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	BACE-1
Manufacturer - Specificity	Mouse Anti-Human Beta-Site APP Cleaving Enzyme 1 (BACE-1) (Clone 137612) recognizes an epitope on Human BACE-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE.
RRID	AB_2828266
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	NS0-Derived Recombinant Human BACE-1 Ectodomain
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Beta-Site APP Cleaving Enzyme 1, 137612, BACE-1, Human, Anti-Human/Mouse BACE-1 Ectodomain, APP beta-secretase, ASP2, Aspartyl protease 2, BACE1, BACE-1, BACEAsp 2, beta-secretase 1 precursor variant 1, beta-secretase 1, beta-site amyloid beta A4 precursor protein-cleaving enzyme, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, beta-site APP-cleaving enzyme 1, beta-site APP-cleaving enzyme, EC 3.4.23, EC 3.4.23.46, FLJ90568, HSPC104, KIAA1149, memapsin-2, Membrane-associated aspartic protease 2, transmembrane aspartic proteinase Asp2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-B596-500ug-safety-datasheet.pdf