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Anti-Human CXCL1 (Clone 20326) - 25 µg

Anti-Human CXCL1 (Clone 20326) - 25 µg

Item no.	LEIN-C1393-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Flow Cytometry Western Blot ELISA Primary Antibodies Immunostaining
Type	Antibody Monoclonal
Applications	WB, FC, ELISA
Clone	20326
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732 2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596 3. Miller, RH. et al. (2002) Cell 110: 373 4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, ELISA Cap, FC, N, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	CXCL1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3, 4
Background	Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).¹ CXCL1 is expressed by macrophages, neutrophils and epithelial cells.² CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.^{3, 4}
Other Applications Reported In Literature	Neutralization: This antibody is useful for neutralization of Human CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CXCL1 (at 10 ng/ml) is 3-15 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	CXCL1
Manufacturer - Research Area	Chemokine
Manufacturer - Specificity	Mouse Anti-Human CXCL1 (Clone 20326) recognizes an epitope on Human CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody exhibits approximately 20% cross-reactivity with rhCXCL2/GROβ and rhCXCL3/GROγ, and no cross-reactivity with other chemokines tested.
RRID	AB_2828450
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human CXCL1 (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, CXCL1, GRO1, GROa, MGSA, MGSA alpha, NAP-3, SCYB1, Clone 20326, Human CXCL1 antibody, Anti-Human CXCL1, GROa antibody, Human GROa, 20326, CINC1, CINC-1, CXCL1, GRO alpha, KC, MGSA-alpha

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1393-25ug-safety-datasheet.pdf