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Anti-Human CD14 – Purified <i>in vivo </i>GOLD™ Functional Grade - 1.0 mg

Anti-Human CD14 – Purified <i>in vivo </i>GOLD™ Functional Grade - 1.0 mg

Item no.	LEIN-C370-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	Neuroscience Neuroinflammation In Vivo Antibodies Flow Cytometry Western Blot Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IV
Clone	UCHM-1
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
NCBI	929
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this UCHM-1 antibody for use in western blotting is 1-10 μg/ml.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD14
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
Background	CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
PubMed	CD14
Ligand/Receptor	LPS
Manufacturer - Research Area	Cell Biology, Immunology, Neuroinflammation, Neuroscience
Manufacturer - Specificity	Clone UCHM-1 recognizes an epitope on human CD14.
RRID	AB_2829682
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Cluster of Differentiation 14, P35, UCHM-1, CD14, Human, Anti-Human CD14 Monocytes Purified Low Endotoxin Functional Formulation, CD-14, CD14 antigen, CD14 molecule, CD14, monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C370-1.0mg-safety-datasheet.pdf