Anti-Human CD14 - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2829682

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this UCHM-1 antibody for use in western blotting is 1-10 μg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone UCHM-1 recognizes an epitope on human CD14.

Antigen Distribution

CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes.

Background

CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Antigen Details

Protein

CD14

Ligand/Receptor

LPS

PubMed

CD14

NCBI Gene Bank ID

929

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

.

Neuroinflammation

.

Neuroscience

References & Citations

1. Ulrich Costabel et al. (1995) CHEST 108: 1614
2. Ulevitch RJ et al. (1993) Immunobiology 187: 227
3. Schumann RR et al. (1994) Medical Microbiol Immunol. 183: 279
4. Verhasselt V et al. (1997) J Immunol. 158: 2919
5. Kirkland TN et al. (2000) J Biol Chem. 275: 3144

Technical Protocols