Anti-Human Ephrin-A4 - 25 µg

Quality Tested by Leinco Western Blotting: To detect Human Ephrin-A4 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human Ephrin-A4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E199.

Ephrin-A4

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Ephrin-A4 is a glycoinositol phospholipid membrane anchor containing ephrin found in variety of adult tissue such as kidney, lung, colon and ovary. Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases.1 Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A4 encodes an EFNA class ephrin. Ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils.

Immunohistochemistry: Suitable for use at concentration of 3-15 µg/mL. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.

Goat Anti-Human Ephrin-A4 recognizes Human Ephrin-A4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Purified Recombinant Human Ephrin-A4 (> 98%)

Polyclonal Antibody, Ephrin-A4, LERK-4, EFL-4, EFNA4, EPLG4, MGC125826, Human Ephrin-A4, Anti-Human Ephrin-A4, EFL4, EFL-4, EFNA4, EPH-related receptor tyrosine kinase ligand 4, EphrinA4, Ephrin-A4, EPLG4MGC125826, LERK-4, LERK4FLJ57652, ligand of eph-related kinase 4