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Anti-Human GITR Ligand

Anti-Human GITR Ligand

Item no.	LEIN-G717-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	FC, other
Clone	109114
Specific against	Human (Homo sapiens)
Host	Mouse
NCBI	8784
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing GITRL. A suggested method would be to stain cells expressing GITR with 25 µg per 1-2.5x 10⁵ cells in a total staining volume of 200 µl followed by PN:M1189. Concentration: 2.5 µg/10⁶ cells
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	GITRL
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	Lyophilized antibodies can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. PBS or reconstituted antibodies can be stored for at least four weeks at 2-8°C and long term storage aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Other Applications Reported In Literature	Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITRL Ligand to immobilized Recombinant Human GITRL/TNFRSF18 Fc Chimera coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block > 90% of the binding. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	GITRL
Manufacturer - Specificity	Mouse Anti-Human Glucocortoid-Induced Tumor necrosis factor Receptor (GITRL) (Clone 109114) recognizes an epitope on Human GITRL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2830288
Formulation	This antibody has been 0.2 µm filtered and formulated in either Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present or lyophilized from Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human GITRL (Accession # Q9UNG2)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-G717-500ug-safety-datasheet.pdf