Anti-Human CCL22

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Chicken

Immunogen

Purified Recombinant Human CCL22 (> 98%)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831362

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human CCL22 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:C1177 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL22 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M171.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human MDC bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human MDC (at 10 ng/ml) is 0.4-2.0 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Chicken Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22) recognizes Human CCL22. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.¹ CCL22 selectively chemo-attract T_h2 cytokine-producing cells^{2, 3} and acts as an important activator of eosinophils once these cells have migrated into tissue.⁴

Antigen Details

Protein

CCL22

PubMed

CCL22

NCBI Gene Bank ID

6367

UniProt.org

More Information on Uniprot.org

References & Citations

1. Sozzani, S. et al. (2001) Eur. J. Immunol. 31: 812
2. Elias, CG. et al. (1998) J Immunol. 161: 5027
3. Gray, PW. et al. (1997) J Exp Med. 185: 1604
4. Teixeira, MM. et al. (2003) J Leuko Biol. 73: 356

Technical Protocols