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Anti-Human PD-ECGF - 100 µg

Anti-Human PD-ECGF - 100 µg

Item no.	LEIN-P264-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Miyazono, K. et al. (1991) Progress in Growth Factor Res. 3: 207 2. Humbert, M. et al. (2008) Am J Respir Crit Care Med. 178: 81
NCBI	1890
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	IHC FFPE, N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	PD-ECGF
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an acidic non-glycosylated protein found in connective tissue of the placenta. It is synthesized by fibroblasts, vascular smooth muscle cells, and a number of transformed cell lines.1 PDGF promotes the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs), and may play a role in the progression of pulmonary arterial hypertension (PAH), a condition characterized by proliferation of PASMCs resulting in the obstruction of small pulmonary arteries.2
Background	Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an acidic non-glycosylated protein found in connective tissue of the placenta. It is synthesized by fibroblasts, vascular smooth muscle cells, and a number of transformed cell lines.¹ PDGF promotes the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs), and may play a role in the progression of pulmonary arterial hypertension (PAH), a condition characterized by proliferation of PASMCs resulting in the obstruction of small pulmonary arteries.²
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human PD-ECGF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human PD-ECGF (at 150 ng/ml) is 0.5-2.0 µg/ml.
PubMed	PD-ECGF
Manufacturer - Specificity	Goat Anti-Human Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) recognizes Human PD-ECGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2831560
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.01 EU/µg as determined by the LAL method
Immunogen	Sf21-derived human PD-ECGF
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, PD-ECGF, Endothelial Cell Growth Factor-1 ECGF-1, Gliostatin, Platelet-Derived Endothelial Cell Migration, PD-ECGF, Human PD-ECGF, Anti-Human PD-ECGF, dThdPase, EC 2.4.2, EC 2.4.2.4, ECGF, ECGF1, ECGF1TP, endothelial cell growth factor 1 platelet-derived, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine Phosphorylase, TPase, TYMP

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-P264-100ug-safety-datasheet.pdf