Human Actin/ACTA1 antibody

Antibodies

Monoclonal antibody

ACTA1 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Cancer

Skeletal muscle, NEM3, NEM2, NEM1, MPFD, CFTDM, CFTD, ASMA, Alpha-actin-1, alpha1, Alpha skeletal muscle actin, Alpha skeletal muscle, Alpha actin 1, alpha 1, Actin, ACTA1, ACTA, ACTA 1, Alpha actin1, NEM 3, NEM 2, NEM 1, Actin, ATGA0517-10ug, ATGA0517-20ug, ATGA0517-50ug, ATGA0517-100ug, ATGA0517-250ug, ATGA0517-500ug, ATGA0517-1mg, ATGA0517-010, ATGA0517-020, ATGA0517-050, ATGA0517-100, ATGA0517-250, ATGA0517-500, ATGA0517-01M

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Lung Tissue lysate
Lane 2.: HeLa cell lysate
Lane 3.: A549 cell lysate
Lane 4.: A431 cell lysate
Lane 5.: Raji cell lysate