TACE Antibody

KO Validation (Figure 2): Anti-TACE antibodies (1131) specificity was further verified by TACE specific knockout. TACE signal was not detected in TACE knockout HeLa cells as compared to that in control wild type cells.

Independent Antibody Validation in Cell lines (Figure 3) shows similar TACE expression profile in human cell lines detected by two independent anti-TACE antibodies that recognize different epitopes, 1131 against C-terminus domain and  22-001 against a synthetic peptide to a sequence within amino acids 700-824 of human TACE.   TACE proteins are detected in most of the tested cell lines except 293 and A549 cell lines at different expression levels by the two independent antibodies.  

KD validation (Figure 6, 7): Anti-TACE antibody (1131) specificity was verified by TACE TACE specific siRNA or shRNA knockdown. TACE signal in COS cells (Figure 6) and MDA-MB-435 cells (Figure 7) with TACE knockdown was disrupted in comparison with control.

Overexpression validation (Figure 8): Anti-TACE antibody (1131) detected high expression levels of TACE in MCF-7 cells transfected with TACE as compared to vector transfected cells.