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Histamine Receptor H1 Antibody

Histamine Receptor H1 Antibody

Manufacturer	ProSci
Category	Food Safety Histamines Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies
Type	Antibody Primary
Specific against	Human
Format	Liquid
Applications	WB, IHC, ELISA
Amount	0.1 mg
Host	Goat
Item no.	PRS-45-732-0.1mg
eClass 6.1	32160702
eClass 9.0	32160702
Available
Shipping	blue ice or RT
By Research Area	Cancer
Immunogen	The immunogen for this antibody is: QQTSVRREDKCETD
Applications	Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4ug/ml.
Specificity	.
Positive Control 1	Cat. No. 1301 - Human Heart Tissue Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-732P - Histamine Receptor H1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HRH1
Accession #	NP_000852.1
Protein Gi #	4504491
Ncbi Gene Id #	3269
Ncbi Official Symbol	HRH1
Ncbi Official Full Name	histamine receptor H1
Ncbi Organism	Homo sapiens
Swissprot #	P35367
Background References 1	Bryce PJ, Mathias CB, Harrison KL, Watanabe T, Geha RS, Oettgen HC. The H1 histamine receptor regulates allergic lung responses. J Clin Invest. 2006 Jun; 116(6):1624-32. Epub 2006 May 4.
1st Image Caption	45-732 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption	45-732 (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 6/6/2024