Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human |
Format |
Liquid |
Applications |
WB, FC |
Amount |
400 ul |
Host |
Rabbit |
Item no. |
PRS-61-995-400ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
Homology |
Predicted species reactivity based on immunogen sequence: Mouse, Rat |
Immunogen |
This TRHDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 951-980 amino acids from the C-terminal region of human TRHDE. |
Applications |
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
117 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TRHDE |
Accession # |
Q9UKU6 |
Protein Gi # |
11387208 |
Ncbi Gene Id # |
29953 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TRHDE |
Ncbi Official Full Name |
Thyrotropin-releasing hormone-degrading ectoenzyme |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UKU6 |
Background |
This gene encodes a member of the peptidase M1 family. TRHDE is an extracellular peptidase that specificallycleaves and inactivates the neuropeptide thyrotropin-releasinghormone. |
Background References 1 |
Cirulli, E.T., et al. Eur. J. Hum. Genet. (2010) In press : |
Background References 2 |
Murabito, J.M., et al. BMC Med. Genet.SUPPL 1, S6 (2007) : |
Background References 3 |
Schomburg, L., et al. Eur. J. Biochem. 265(1):415-422(1999) |
Background References 4 |
Bodeker, H., et al. Mol. Cell Biol. Res. Commun. 2(3):150-154(1999) |
1st Image Caption |
Western blot analysis in MDA-MB435 cell line lysates (15ug/lane). |
2nd Image Caption |
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.