Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Mouse, Rat |
Format |
Liquid |
Applications |
WB, IF, IHC |
Amount |
0.1 mg |
Host |
Rabbit |
Item no. |
PRS-79-276-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
ATF2 (Ab-62 or 44) antibody was raised against a peptide sequence around aa.serine 60~64 or 42~46 (N-D-S-V-I) derived from Human ATF2. |
Applications |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
Specificity |
This antibody detects endogenous level of total ATF-2 protein. |
Predicted Molecular Weight |
65 - 75 kDa |
Purification |
Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration |
1 mg/mL |
Storage Conditions |
Store antibody at -20˚ C for up to one year. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ATF2 |
Accession # |
NP_001871.2 |
Protein Gi # |
22538422 |
Ncbi Gene Id # |
1386 |
Ncbi Official Symbol |
ATF2 |
Ncbi Official Full Name |
activating transcription factor 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P15336 |
Background |
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. |
Background References 1 |
Sevilla A, et al. (2004) J Biol Chem. 279 (26): 27458-27465. Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181 (2): 629-635. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8): 1798-1811. |
1st Image Caption |
Western blot analysis of lysed extracts from 293 and 3T3 cells using ATF2 (Ab-62 or 44). |
2nd Image Caption |
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Ab-62 or 44).Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Ab-62 or 44). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.