Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human |
Format |
Liquid |
Applications |
WB, IF, IHC, ELISA |
Amount |
0.1 mg |
Host |
Rabbit |
Item no. |
PRS-8657-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Homology |
Predicted species reactivity based on immunogen sequence: Rat: (80%) |
Immunogen |
LAG3 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human LAG3.
The immunogen is located within amino acids 470 - 520 of LAG3. |
Applications |
LAG3 antibody can be used for Western blot at 1 μ g/mL. Antibody can also be used for Immunohistochemistry at 2 μ g/mL. For Immunoflorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1304 - Human Liver Tissue Lysate |
Positive Control 2 |
Cat. No. 11-533 - Human Tonsil Tissue Slide |
Predicted Molecular Weight |
Predicted: 55 kDa
Observed: 40 kDa |
Purification |
LAG3 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
LAG3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
LAG3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 8657P - LAG3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
LAG3 |
Accession # |
NP_002277 |
Protein Gi # |
167614500 |
Ncbi Gene Id # |
3902 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
LAG3 |
Ncbi Official Full Name |
lymphocyte activating 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P18627 |
Background |
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5). |
Background References 1 |
Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21. |
Background References 2 |
Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22. |
Background References 3 |
Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91. |
Background References 4 |
Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9. |
1st Image Caption |
Western blot analysis of LAG3 in human liver tissue lysate with LAG3 antibody at (A) 1 and (B) 2 μ g/mL |
2nd Image Caption |
Immunohistochemistry of LAG3 in human tonsil tissue with LAG3 antibody at 2 μ g/ml. |
3rd Image Caption |
Immunofluorescence of LAG3 in human tonsil tissue with LAG3 antibody at 20 μ g/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.