Comparison

EnzyChrom Acetylcholine Assay Kit

Item no. BAS-EACL-100
Manufacturer BioAssay Systems
Amount 100 Tests
Quantity options 100 Tests 100x100 Tests 20x100 Tests
Category
Type Assay-Kit
Specific against other
Citations Ahmad, A., Ramasamy, K., Jaafar, S. M., Majeed, A. B. A., & Mani, V. (2014). Total isoflavones from soybean and< i> tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain. Food and Chemical Toxicology 65: 120-128. Assay: Acetylcholine in rat brain.

Baitharu, I., Jain, V., Deep, S. N., Hota, K. B., Hota, S. K., Prasad, D., & Ilavazhagan, G. (2013). Withania somnifera root extract ameliorates hypobaric hypoxia induced memory impairment in rats. Journal of ethnopharmacology 145(2): 431-441. Assay: Acetylcholine in rat hippocampus.

Chen L et al (2012). Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae. Environ Toxicol Chem. 31(10):2338-44. Assay: Acetylcholine in fish.

He C, et al (2012). Embryonic exposure to benzo(a)pyrene influences neural development and function in rockfish (Sebastiscus marmoratus). Neurotoxicology 33(4):758-62. Assay: Acetylcholine in fish.

He C, et al (2012). Exposure of Sebastiscus marmoratus embryos to pyrene results in neurodevelopmental defects and disturbs related mechanisms. Aquat Toxicol 15(116-117):109-15. Assay: Acetylcholine in fish.

Mani V, et al (2012). Effects of the Total Alkaloidal Extract of Murraya koenigii Leaf on Oxidative Stress and Cholinergic Transmission in Aged Mice. Phytother Res. 27(1):46-53. Assay: Acetylcholine in mouse brain.


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ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Available
Description
Quantitative determination of acetylcholine by colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 30 min. Kit size: 100 tests. Detection limit: colorimetric assay 10 uM, fluorimetric assay 0.4 uM. Shelf life: 3 months. Shipping: on ice
Faqs

Can the acetylcholine level in human serum be measured with this kit?

We have not tested this assay with blood samples. In the literature search I found the following reference:

Plasma levels in young women: 456.1 +/- 53.1 (SEM) pg/mL which equals 3.1 nM. (Kawashima et al. Neuroscience Letters 80:(3), 339-342; 1987.) If this is indeed a representative study, it would mean that our assay is not sensitive enough to detect acetylcholine in plasma or serum samples. Furthermore, plasma levels of choline are much higher (ca.10 & microM) and will thus have to be removed before assaying.

What is the principle of this assay?

The assay is based on the enzymatic degradation of acetylcholine to choline and acetate and the subsequent detection of choline.

Can this assay measure acetylcholine in brain tissue?

We have not tested acetylcholine assay specifically with brain tissue samples, but believe it should work. Typical concentrations of choline and acetylcholine in brain tissue are 10-70 nmoles/g. So if the tissue sample is suspended in ice-cold PBS at 500 mg/mL, we should expect 5-35 ?M choline or acetylcholine in the supernatant.

Suggested protocol: weigh brain tissue (approx. 50 mg) and add 100 uL ice-cold PBS, lyze tissue by homogenization or sonication. Centrifuge at 14, 000 rpm for 10 min at 4C. Carefully transfer the supernatant to a clean tube and perform the assays for choline and acetylcholine.

Due to the low concentration range, the fluorescence assay procedure is recommended.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview

Application

  • For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.

Key Features

  • Use 20 ?L samples. Linear detection range: colorimetric assay 10 to 200 ?M, fluorimetric assay 0.4 to 10 ?M acetylcholine.

Method

  • OD570nm, or FL530/585nm

Samples

  • Serum, plasma, urine, saliva, milk, tissue, and cell culture etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • OD, FL: 10, 0.4 ?M

Shelf Life

  • 6 months

More Details

  • ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
Shipment
ICE
Storage
-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 Tests
Available: In stock
available

Delivery expected until 9/25/2025 

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