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EnzyChrom Choline Assay Kit

EnzyChrom Choline Assay Kit

Item no.	BAS-ECHO-100
Manufacturer	BioAssay Systems
Amount	100 Tests
Quantity options	100 Tests 100x100 Tests 20x100 Tests
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Citations	Winnard Jr, PT et al (2020). Brain metabolites in cholinergic and glutamatergic pathways are altered by pancreatic cancer cachexia. Journal of Cachexia, Sarcopenia and Muscle, 11(6), Assay: Choline in mouse plasma. Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma. Benedito-Palos, L et al (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Choline in fish plasma. Ballester-Lozano, GF et al (2014). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma. Strilakou, AA et al (2014). Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats. Canadian Journal of Physiology and Pharmacology 92(1): 78-84. Assay: Choline in rat serum. To find more recent publications, please click here.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Description	Quantitative determination of choline by colorimetric (570nm) or fluorometric (530nm/590nm) methods. Procedure: 30 min. Kit size: 100 tests. Detection limit: colorimetric assay 1 uM, fluorimetric assay 0.2 uM. Shelf life: 3 months. Shipping: on ice; stora
Faqs	What are known interferences? Chondroitin sulfate C (chondroitin-6-sulfate) appears to interfere with the assay; creating false positive results. For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview	Application For quantitative determination of choline and evaluation of drug effects on choline metabolism. Key Features Use 20 ?L samples. Linear detection range: colorimetric assay 1 to 100 ?M, fluorimetric assay 0.2 to 10 ?M choline. Method OD570nm, or FL530/585nm Samples Serum, plasma, urine, saliva, milk, tissue, cells etc Species All Size 100 tests Detection Limit OD, FL: 1, 0.2 ?M Shelf Life 6 months More Details CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to ?ine and H₂O₂ which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
Shipment	ICE
Storage	-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 Tests

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Delivery expected until 9/25/2025

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