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RayPlex Mouse Inflammation Bead Array 1

RayPlex Mouse Inflammation Bead Array 1

Item no.	FAM-INF-1-480
Manufacturer	Raybiotech
Amount	480 tests
Quantity options	192 tests 48 tests 480 tests 96 tests
Category	Arrays Antibody Arrays
Type	Array
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Multiplexed Protein Detection Biomarker Screening Identifying Key Factors Confirming Biological Process Biomarker Validation Validation of Antibody Array Results Quantitative Protein Detection Establishing Normal Range
Manufacturer - Category	Arrays\|Cytometric Bead Arrays\|Antibody Arrays\|Inflammation Arrays
Manufacturer - Targets	IFN-gamma, IL-1 beta, MCP-1, TNF alpha, IL-10, IL-17A, IL-2, IL-4, GRO alpha, IL-6, IL-12 p70, GCSF, IL-23 p19
Shipping Temperature	Blue ice
Storage Conditions	4°C
Research Area	Inflammation
Description	This RayPlex® kit is a cytometric bead array to quantitatively measure, by flow cytometry, 13 key mouse inflammatory cytokines. The assay has been optimized for serum and cell culture supernatant.
Number of Targets Detected	13
Compatible Sample Types	Cell Culture Supernatants, Serum
Design Principle	Sandwich-based
Method of Detection	Flow Cytometry
Quantitative/Semi-Quantitative	Quantitative
Solid Support	Fluorescent Microbeads
Gene Symbols	Ccl2 \| Csf3 \| Cxcl1 \| Ifng \| Il10 \| Il12a \| Il17a \| Il1b \| Il2 \| Il23a\| Il4 \| Il6 \| TNF
Kit Components	RayPlex„¢ Multiplex Bead Cocktail1 X Assay Diluent20 X Wash Buffer Lyophilized Protein Standard Mix10 X Biotinylated Detection Antibody Cocktail10 X Streptavidin-PEFlow Cytometer Setup Bead Cocktail V-bottom 96-well Microplate Filter 96-well Microplate Ray Bio® Serum Diluent
Other Materials Required	Orbital 96-well plate shaker (with ability to reach 1000 rpm) Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (AP C) channels Aluminum foil or 96-well Plate Aluminum Sealers Distilled water 1.5 ml polypropylene microcentrifuge tubes or similar Microcentrifuge If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate Optional: 96-well round bottom plate (to prepare samples) Optional: High throughput sampler (HT S) for 96-well plate reading Don't have a compatible flow cytometer? Ray Biotech provides full testing services for RayPlex„¢ bead arrays. Get a quote!
Protocol Outline	Prepare Reagents, Standards, and Samples Add RayPlex„¢ Multiplex Bead Cocktail to each well Incubate with Samples and Standards Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluorophore Analyze with a compatible flow cytometer Perform data analysis
UNSPSC Code	41116133

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.