Comparison

Anti-Myelin Basic Protein (MBP) Antibody

Item no. MBP
Manufacturer Aves Labs
Amount 1000 uL
Quantity options 1000 uL 100 uL
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IHC, ICC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Chicken
Isotype IgY
Conjugate/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Cell Markers, Glial and Schwann Cell Markers
Manufacturer - Targets
Myelin Basic Protein (MBP)
Country of Origin
United States
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Molecular Weight
21, 18, 17, 14 kDa
Concentration
100 µg/mL
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Description
An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.
Manufacturer - Statement
We produce our Anti-Myelin Basic Protein (MBP) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.
Target Description
Human Myelin Basic Protein (MBP) is a 20, 115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.
Antibody Registry ID (RRID)
AB_2313550
Clonality
Polyclonal
Dilution Range ICC
1:1000-1:2000
Dilution Range IHC
1:1000-1:2000
Dilution Range Western Blot
1:1000-1:2000
Immunogen
Synthetic peptide
Immunogen Species
Human, Mouse, Rat
Manufacturer - Form
Affinity-Purified IgY
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1000 uL
Available: In stock
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