MAb to EGFR activated pTyr1173 Antibody (0.1mg)

Immunoblotting: 0.5 ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. munoprecipitation: 1-10ug per 106 vanadate treated A431 cells. Immunocytochemistry: 1-10ug/ml ELISA: 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Aliquot and store at -80C. Thawed aliquots may be stored at 2-8C for up to 3 months.

Epidermal Growth Factor Receptor (EGFR), phospho-Tyr 1173 & Positive Control Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and tophosphorylation on tyrosine residues, Y1173 being the major autophosphorylation site. Clone 9H2 specifically interacts with the 1170 - NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. This phosphorylation site-specific monoclonal antibody allows the detection and quantification of activated EGFR in crude cell extracts in Immunoblot, ELISA and Biosensor assay formats without the use of P32. Reacts with Human, mouse, and dog.