Anti-Tyrosine Hydroxylase

Polyclonal Antibody

Wet Ice

The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF.
Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

Tyrosine 3-monooxygenase

Prepared from rabbit serum by affinity purification using a protein A column and using a column to which the immunogen was coupled.

Most mammals, Some invertebrates