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DHFR Protein (OPPA02042)

DHFR Protein (OPPA02042)

Manufacturer	AVIVA Systems Biology
Category	Oncology By Cell Type Blastoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	other
Format	The DHFR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M Nacl 2mM DTT, and 30% glycerol. Physical appearance: Sterile Filtered colorless solution.
Amount	10ug
Host	E.coli
Item no.	OPPA02042
eClass 6.1	34160400
eClass 9.0	42020190
Available
Gene symbol	DHFR
Protein name	Dihydrofolate reductase
Product format	The DHFR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M Nacl 2mM DTT, and 30% glycerol. Physical appearance: Sterile Filtered colorless solution.
Gene id	1719
Reconstitution and storage	Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Description of target	DHFR Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.6kDa.The DHFR is purified by proprietary chromatographic techniques.
Biological activity	Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 umole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25C.
Purity	Greater than 95.0% as determined by SDS-PAGE.
Drywet	Wet Ice

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