Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IHC |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BS3485-100 |
Targets |
ACE |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
P12821 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exo-peptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
ACE (F940) pAb detects endogenous levels of Angiotensin-converting enzyme protein. |
WB |
Western blot (WB) analysis of ACE (F940) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:PC12 cell lysate Lane3:Mouse kidney tissue lysate Lane4:Rat liver tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of ACE (F940) pAb in paraffin-embedded human brain tissue. |
BiowMW |
Predicted band size:150KDa Observed band size:195KDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Angiotensin-converting enzyme, Short name=ACE, Dipeptidyl carboxypeptidase I, CD143, ACE1, DCP, DCP1, Angiotensin Converting Enzyme 1 |
Immunogen |
Synthetic peptide, corresponding to amino acids 920-950 of Human ACE. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.