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Anti-Beclin-1 BECN1 Antibody

Anti-Beclin-1 BECN1 Antibody

Item no.	BOS-A00327-1
Manufacturer	Boster
Amount	0.1 mg
Quantity options	0.1 mg 0.1 mg
Category	Cell Biology Cell Status Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Autophagy Immunostaining Immunochemistry
Format	Liquid
Applications	WB, IF, ICC, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Alias	ATG6,VPS30,beclin1,GT197,Beclin-1,Coiled-coil myosin-like BCL2-interacting protein
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Beclin-1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	51896 MW
Clonality	Polyclonal
Application Details	WB: 1-2 μg/mL IHC-P: 2-5 μg/mL ICC/IF: 10-20 μg/mL IHC-P/IF: 10-20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
Gene Name	BECN1
Gene Full Name	Beclin 1, autophagy related
Background	Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Immunogen	Anti-Beclin-1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human Beclin-1. The immunogen is located between 40-90 amino acids of Beclin-1.
Contents	Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	Beclin-1 Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Apoptosis, Autophagy, Cancer
Protein Name	Beclin 1, autophagy related
Protein Function	Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Subcellular Localization	Cytoplasm
Description	Boster Bio Anti-Beclin-1 BECN1 Antibody (Catalog # A00327-1). Tested in ELISA, WB, ICC/IF, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Ubiquitous.
Short Description	Boster Bio Anti-Beclin-1 BECN1 Antibody (Catalog # A00327-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Predicted Reactivity	Bovine, Pig

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A00327-1-datasheet.pdf

BOS-A00327-1-safety-datasheet.pdf