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Anti-Survivin BIRC5 Antibody

Anti-Survivin BIRC5 Antibody

Item no.	BOS-A00379-1
Manufacturer	Boster
Amount	0.1 mg
Quantity options	0.1 mg 100 ul 100 ul
Category	Oncology Neuroscience Neural Lineage Markers By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Melanoma Cell Biology Cell Status Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Autophagy Immunostaining Neuronal Cell Markers Immunochemistry
Format	Liquid
Applications	WB, IF, ICC, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
Isotype	IgG
Alias	API4,EPR-1,API4,IAP4,Baculoviral IAP repeat-containing protein 5,Apoptosis inhibitor 4
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Survivin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	16389 MW
Clonality	Polyclonal
Application Details	WB: 1-5μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL; IHC: 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	BIRC5
Gene Full Name	Baculoviral IAP repeat-containing 5
Background	Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Immunogen	Anti-Survivin antibody was raised against a peptide corresponding to 12 amino acids near the amino terminus of human Survivin. The immunogen is located within the first 50 amino acids of Survivin.
Contents	Survivin Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	Survivin Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Apoptosis, Cancer
Protein Name	Baculoviral IAP repeat-containing 5
Protein Function	Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
Subcellular Localization	Cytoplasm
Description	Boster Bio Anti-Survivin BIRC5 Antibody (Catalog # A00379-1). Tested in ELISA, WB, ICC, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Tissue Specificity	Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126).
Predicted Reactivity	Pig, Bovine, Rat

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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BOS-A00379-1-datasheet.pdf

BOS-A00379-1-safety-datasheet.pdf