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Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody

Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody

Item no.	BOS-M19774
Manufacturer	Boster
Amount	100 ug
Category	Oncology Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Hematology Leukemia Lymphoma Immunohistochemistry Immunofluorescence Primary Antibodies By Organ Skin Cancer Renal Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Clone	SPM286
Specific against	Human (Homo sapiens), Horse (Equine)
Host	Mouse
Isotype	IgG2b Kappa
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Melanocyte protein PMEL;ME20-M;ME20M;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;Premelanosome protein;Silver locus protein homolog;M-alpha;95 kDa melanocyte-specific secreted glycoprotein;P26;Secreted melanoma-associated ME20 antigen;ME20-S;ME20S;M-beta;PMEL;D12S53E, PMEL17, SILV;
Available
Manufacturer - Category	Primary Antibodies, Monoclonal Antibodies, 2279
Storage Conditions	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Observed Molecular Weight	70255 MW
Clonality	Monoclonal
Application Details	Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Manufacturer - Gene Name	SILV
Gene Full Name	Melanocyte protein PMEL
Background	By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This monoclonal antibody labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Immunogen	Membranes from a human melanoma metastasis
Contents	Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purification	200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.
Concentration	Purified antibody with BSA and azide at 200ug/ml
Manufacturer - Research Category	Amino Acid Metabolism, Amino Acids, Cancer, Cell Type Markers, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Tumor Associated, Tumor Immunology, Tumor-Associated Antigens
Protein Function	Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Subcellular Localization	Cytoplasmic
Short Description	Boster Bio Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody (Catalog # M19774). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Horse.
Description	Boster Bio Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody (Catalog # M19774). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Horse.
Tissue Specificity	Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M19774-datasheet.pdf

BOS-M19774-safety-datasheet.pdf