Manufacturer |
Cell Sciences
|
Category |
|
Type |
Antibody Monoclonal |
Specific against |
Human |
Clone |
S5 |
Isotype |
IgG1 |
Applications |
WB, IHC |
Amount |
100 ug |
Host |
Mouse |
Item no. |
CS-CMH115 |
Targets |
HSPA4 |
eClass 6.1 |
32160702 |
eClass 9.0 |
42030590 |
Available |
|
Application Notes |
Western blot analysis (1 ug/ml), immunoprecipitation (5-10 ug/ml), Immunohistochemistry (5-20 ug/ml, frozen & paraffin), and FACS analysis. However, the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73). |
Concentration |
0.5 mg/ml |
Cross-Reactivity |
Reacts with human, mouse, rat, monkey, cow, rabbit, hamster, guinea pig, dog, sheep, chicken, pig. Does not cross-react with the constitutive HSPA8. |
Description |
Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP, in vitro , but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus, b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus, c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum, and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. |
Formulation |
Liquid in PBS + 1 mg/ml BSA + 1.5 mM sodium azide + 50% glycerol. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only. |
Immunogen |
Recombinant human Hsp70 |
Reconstitution |
The antibody solution should be gently mixed before use. The optimal concentration should be determined by the user for each specific application. |
Storage and Stability |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
Specificity |
The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.