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Rabbit Anti-Human Mcl-1 Affinity Purified pAb

Rabbit Anti-Human Mcl-1 Affinity Purified pAb

Manufacturer	Cell Sciences
Category	Oncology Antibodies By Cell Type Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Western Blot Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human
Isotype	IgG
Applications	WB
Amount	100 ug
Host	Rabbit
Item no.	CS-CPM110
Targets	MCL1
eClass 6.1	32160702
eClass 9.0	32160702
Available
Application Notes	For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunoprecipitation, use a working dilution of 10-20 ug/ml.For Immunohistochemistry, use a working dilution of 10-20 ug/ml.Blocking Peptide (Cat. No. CPM110BP) is available separately.The optimal concentration should be determined by the user for each specific application.
Concentration	0.2 mg/ml
Cross-Reactivity	Human, Mouse, Rat, Monkey
Description	Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-a1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Formulation	100 ug (0.2 mg/ml) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen	Synthetic peptide corresponding to residues surrounding amino acids 131 of human MCL1
Purification	Affinity purified
Storage and Stability	Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CS-CPM110-datasheet.pdf

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Amount: 100 ug

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Delivery expected until 5/23/2024