Rabbit Anti-Human RAF1 Affinity Purified pAb

0.2 mg/ml

Rabbit Anti-Human RAF1 Affinity Purified polyclonal antibody. Human RAF1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of MEK1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (MEK1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. RAF1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in RAF1. RAF1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. RAF1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2), and the catalytic domain (CR3). RAF1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins, and the molecular chaperones hsp90 and p50.

Synthetic peptide mapping to the C-terminus of human RAF1

Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.