BT-20 cells

Disease:

Carcinoma

Origin:

This breast tumor line was established in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. ¬

Species:

Homo sapiens

Tissue:

Mammary gland/breast

Morphology:

Epithelial;

Properties:

Adherent

Cytogenic data:

Normal chromosomes N3, N4, N9, N13, N14, and X may be absent. The markers der(11)t(11; ?)(q25; ?) (M1); der(1)t(1; 3)(p22?; p13?) (M2); and der(2)t(2; ?) (q37; ?) (M5) were detected

Patient:

74 year old Caucasian, female

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture at 1:2 to 1:4; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO