BT-20 cells

Disease:
Carcinoma
Origin:
This breast tumor line was established in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. ¬
Species:
Homo sapiens
Tissue:
Mammary gland/breast
Morphology:
Epithelial;
Properties:
Adherent
Cytogenic data:
Normal chromosomes N3, N4, N9, N13, N14, and X may be absent. The markers der(11)t(11; ?)(q25; ?) (M1); der(1)t(1; 3)(p22?; p13?) (M2); and der(2)t(2; ?) (q37; ?) (M5) were detected
Patient:
74 year old Caucasian, female
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Subculture at 1:2 to 1:4; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11, 14
D5S818: 12
D7S820: 10
THO1: 7, 9.3
TPOX: 11
vWA: 16, 17
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: BT-20 (AddexBio C0006014)
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines