Comparison

AGS cells

Item no. C0023004
Manufacturer Addexbio
Amount One Frozen vial
Category
Type Tumour Cells
Specific against Human
Dry ice Yes
Citations 1. Barranco SC, Townsend CM Jr, Quraishi MA, Burger NL, Nevill HC, Howell KH, Boerwinkle WR. Heterogeneous responses of an in vitro model of human stomach cancer to anticancer drugs. Invest New Drugs. 1983;1(2):117-127. <a href="http://www.ncbi.nlm.nih.gov/pubmed/ 6678861 "> PMID: 6678861 </a>
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41106509
Available
Description

Disease:

Gastric adenocarinoma

Origin:

This cell line was derived from fragments of the adenocarcinoma of the stomach from a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

Species:

Homo sapiens, human

Tissue:

Stomach

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Hyperdiploid. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1; 8) (q12; p23), der(19)t(19; ?) (q13.6; ?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Patient:

Female, Caucasian, 54 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

II [AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5)]

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

DNA Profile (STR):

Amelogenin: X

CSF1PO: 11, 12

D13S317: 12

D16S539: 11, 13

D5S818: 9, 12

D7S820: 10, 11

THO1: 6, 7

TPOX: 11, 12

vWA: 16, 17

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: AGS (AddexBio C0023004)

Storage condition
LN2
Manufacturers Category
Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: One Frozen vial
Available: In stock
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