Vero E6 Cells

Vero E6 cells, also known as Vero C1008 or Vero 76 clone E6, are a continuous line of epithelial cells derived from the kidney of the African green monkey, Chlorocebus sabaeus. The Vero clone E6, a subline of Vero cells, is particularly noted for its utility in virology research due to its high susceptibility to a wide range of viruses, including coronaviruses like SARS-CoV and SARS-CoV-2, the Ebola virus, and the Zika virus.
The cell line is crucial in the production of vaccines, such as those for Japanese Encephalitis vaccine, due to their capacity for virus culture and isolation. The cells have played a pivotal role in the development of COVID therapeutics, including the testing of the polymerase inhibitor remdesivir. With their ability to support the replication of a variety of viruses, Vero E6 cells facilitate compound screening and the evaluation of antiviral efficacy.
Their role in clinical trials extends to the assessment of anti-inflammatory drugs like dexamethasone and the study of gene products like the P-glycoprotein (pgp protein) encoded by the pgp gene. Vero E6 cells lack the interferon-β gene, which partly explains their high susceptibility to viral infections; this deficiency prevents them from mounting an effective innate antiviral response.
In summary, Vero E6 cells are a valuable resource in the field of virology and biomedicine, providing a versatile platform for antiviral screening, the study of replication in Vero, and aiding in the quest for understanding retroviral sequences.

Adherent

Epithelial

EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

22 hours