« Back
Home /
Recombinant Human Tumor necrosis factor protein(TNF),partial (Active)

Recombinant Human Tumor necrosis factor protein(TNF),partial (Active)

Item no.	CSB-AP002141HU-500
Manufacturer	Cusabio
Amount	500 ug
Quantity options	1 mg 10 ug 100 ug 250 ug 50 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized powder
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Purity	>97% as determined by SDS-PAGE and HPLC.
Sequence	MHHHHHH+VRS SSRTPSDKPV AHVVANPQAE G QLQWLNRRA NALLANGVEL RDNQLVVPSE GLY LIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQ TKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYL GGV FQLEKGDRLS AEINRPDYLD FAESGQVYF G IIAL
Protein Family	Tumor necrosis factor family
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, , NTF
Available
Manufacturer - Category	Cytokine / Tumor Necrosis Factor
Manufacturer - Conjugate / Tag	N-terminal 6xHis-tagged
Molecular Weight	18.3 kDa
General Research Areas	Cancer
Relevance	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). {ECO:0000269\|PubMed:16829952, ECO:0000269\|PubMed:22517918, ECO:0000269\|PubMed:23396208}.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000269\|PubMed:16829952}.
Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective
Subcellular Location	Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted
Involvement in disease	Psoriatic arthritis (PSORAS)
Paythway	MAPKsignalingpathway
Gene Names	TNF, TNFA, TNFSF2
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Storage Buffer	0.2 m filtered PBS, pH 7.0 , lyophilized
Uniprot Entry Name	TNFA_HUMAN
Expression Region	77-233aa
Protein Description	Partial
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of ? 2.0 x 107 IU/mg in the presence of actinomycin D.
PubMed ID	3555974; 6392892; 3883195; 2995927; 3856324; 3932069; 8499947; 10202016; 14656967; 15489334; 8631363; 8597870; 10205166; 2009860; 1402651; 9034191; 10369255; 16829952; 16829951; 22517918; 23396208; 2922050; 1964681; 2551905; 9488135; 9442056; 12746914; 12915457

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CSB-AP002141HU-500-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 500 ug

available

Delivery expected until 8/28/2025

Express Delivery: Save 3 days

Compare

Add to wishlist

Get an offer