Comparison

Recombinant Human Proteasome subunit beta type-3(PSMB3)

Manufacturer Cusabio
Category
Type Proteins
Specific against other
Amount 1mg
Host E.coli
Item no. CSB-EP018880HU-1
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Topic
Cell Biology
Uniprot ID
P49720
Gene Names
PSMB3
Organism
Homo sapiens (Human)
AA Sequence
SIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVT TDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRL NLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTE PVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGT CAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVD RDAVSGMGVIVHIIEKDKITTRTLKARMD
Expression Region
1-205aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
49.8 kDa
Alternative Name
Proteasome chain 13
Proteasome component C10-II
Proteasome theta chain
Relevance
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Reference
"Sequence analyses and inter-species comparisons of three novel human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine potential proteolytic active-site residues."
Nothwang H.G., Tamura T., Tanaka K., Ichihara A.
Biochim. Biophys. Acta 1219:361-368(1994)
Purity
Greater than 90% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Subcellular Location
Cytoplasm, Nucleus
Protein Families
Peptidase T1B family
Amount: 1mg
Available: In stock
Listprice: €1,552.27
Price: €1,552.27
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