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Recombinant Ambrosia artemisiifolia Pectate lyase 5

Recombinant Ambrosia artemisiifolia Pectate lyase 5

Item no.	CSB-EP329754BYC-1
Manufacturer	Cusabio
Amount	1mg
Quantity options	1mg 10ug 100ug 20ug 200ug 50ug 500ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Liquid or Lyophilized powder
Specific against	other
Host	E.coli
Purity	Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Available
Research Areas	Others
Target / Protein	N/A
Biologically Active	Not Test
Expression System	E.coli
Species of origin	Ambrosia artemisiifolia (Short ragweed)
Uniprot ID	P27759
AA Sequence	AEDLQEILPVNETRRLTTSGAYNIIDGCWRGKADW AENRKALADCAQGFGKGTVGGKDGDIYTVTSELDD DVANPKEGTLRFGAAQNRPLWIIFERDMVIRLDKE MVVNSDKTIDGRGAKVEIINAGFTLNGVKNVIIHN INMHDVKVNPGGLIKSNDGPAAPRAGSDGDAISIS GSSQIWIDHCSLSKSVDGLVDAKLGTTRLTVSNSL FTQHQFVLLFGAGDENIEDRGMLATVAFNTFTDNV DQRMPRCRHGFFQVVNNNYDKWGSYAIGGSASPTI LSQGNRFCAPDERSKKNVLGRHGEAAAESMKWNWR TNKDVLENGAIFVASGVDPVLTPEQSAGMIPAEPG ESALSLTSSAGVLSCQPGAPC
Tag Info	N-terminal 6xHis-SUMO-tagged
Expression Region	26-396aa
Protein Length	Full Length of Mature Protein
MW	55.8 kDa
Relevance	Has pectate lyase activity.
Reference	Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen.Rafnar T., Griffith I.J., Kuo M.-C., Bond J.F., Rogers B.L., Klapper D.G.J. Biol. Chem. 266:1229-1236(1991)
Purity	Greater than 90% as determined by SDS-PAGE.
Form	Liquid or Lyophilized powder
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Has pectate lyase activity.
Protein Families	Polysaccharide lyase 1 family, Amb a subfamily
Tissue Specificity	Pollen and flowers.
Tag Information	N-terminal 6xHis-SUMO-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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