Comparison

Recombinant Ba(E.coli-k12) KHG/KDPG aldolase(eda)

Item no. CSB-EP359248ENT-100
Manufacturer Cusabio
Amount 100 ug
Quantity options 1 mg 10 ug 100 ug 200 ug 50 ug 500 ug
Category
Type Proteins Recombinant
Specific against Escherichia coli (E.coli)
Host E.coli
Conjugate/Tag HIS
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKA LVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVG AGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAAT EGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEAN GGVKALQAIAGPFSQVRFCPTGGISPANYRDYLAL KSVLCIGGSWLVPADALEAGDYDRITKLAREAVEG AKL
Citations Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias 2-keto-4-hydroxyglutarate aldolase ;KHG-aldolase2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14) ;2-keto-3-deoxy-6-phosphogluconate aldolase ;KDPG-aldolasePhospho-2-dehydro-3-deoxygluconate aldolasePhospho-2-keto-3-deoxygluconate aldolase
Available
Manufacturer - Conjugate / Tag
N-terminal 6xHis-tagged
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight
MW of Fusion: 26, 3
Relevance
Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.
Expression Region
1-213aa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Gene Names
eda
Sequence Info
Full Length
Storage Buffer
Tris-based buffer, 50% glycerol

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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