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Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE(tsaE)

Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE(tsaE)

Item no.	CSB-EP360227ENV-50
Manufacturer	Cusabio
Amount	50 ug
Quantity options	1 mg 10 ug 100 ug 20 ug 200 ug 50 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	other
Host	E.coli
Conjugate/Tag	HIS
Purity	Greater than 90% as determined by SDS-PAGE.
Sequence	MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYG DLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYT LDNLMVYHFDLYRLADPEELEFMGIRDYFANDAIC LVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAV SSAGELLLARLAG
Protein Family	TsaE family
Citations	"Conserved network of proteins essential for bacterial viability."Handford J.I., Ize B., Buchanan G., Butland G.P., Greenblatt J., Emili A., Palmer T.J. Bacteriol. 191:4732-4749(2009).
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
Available
Manufacturer - Conjugate / Tag	N-terminal 6xHis-tagged
Storage Conditions	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight	20.9 kDa
Buffer	Tris-based buffer, 50% glycerol
General Research Areas	Streptomyces viridochromogenes
Relevance	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Expression Region	1-153aa
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Subcellular Location	Cytoplasm
Gene Names	tsaE
Sequence Info	Full Length
Organism	Escherichia coli (strain K12)
Manufacturer - Format	Liquid or Lyophilized powder

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CSB-EP360227ENV-50-datasheet.pdf

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Amount: 50 ug

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Delivery expected until 11/13/2025

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