Comparison

Recombinant Human Guanine nucleotide-binding protein subunit alpha-13(GNA13)

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 10ug
Item no. CSB-EP618885HU-10
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Topic
Signal Transduction
Uniprot ID
Q14344
Gene Names
GNA13
Organism
Homo sapiens (Human)
AA Sequence
MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEID KCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRI IHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREK LHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETR VFLQYLPAIRALWADSGIQNAYDRRREFQLGESVK YFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYD FEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILF LVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRV FSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGD PHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAI NTENIRLVFRDVKDTILHDNLKQLMLQ
Expression Region
1-377aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal 6xHis-tagged
MW
48.0 kDa
Relevance
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1).Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation
Reference
"Leukemia-associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G(12) family to Rho."
Fukuhara S., Chikumi H., Gutkind J.S.
FEBS Lett. 485:183-188(2000)
Purity
Greater than 85% as determined by SDS-PAGE.
Storage Buffer
Tris-based buffer, 50% glycerol
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems
Subcellular Location
Cell membrane, Lipid-anchor, Melanosome, Cytoplasm, Nucleus
Protein Families
G-alpha family, G(12) subfamily
Tissue Specificity
Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).
Paythway
Regulationofactincytoskeleton
Tag Information
N-terminal 6xHis-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 10ug
Available: In stock
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