Comparison

COVID-19 Human IgG ELISA Kit

Manufacturer Raybiotech
Category
Type Elisa-Kit
Specific against other
Amount 5 Plate Kit
Host Hamster - Armenian
Item no. IEQ-CoVS1RBD-IgG-5
eClass 6.1 32160605
eClass 9.0 32160605
Available
Species Detected
Human
Compatible Sample Types
Dried Blood
Plasma
Saliva
Serum
Design Principle
Indirect
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
IGG
Kit Components
S
A
R
S-
Co
V-2 N-protein coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Anti-
Human Ig
G
H
R
P-
Streptavidin concentrate
T
M
B One-
Step Substrate
Stop Solution
Assay Diluent5
X Sample Diluent1
X P
B
S (
D
B
S version)
Tween-20 (
D
B
S version)
Protease Inhibitor Cocktail (
D
B
S version)
Other Materials Required
Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Distilled or deionized water
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Biotinylated Anti-
Human Ig
G Antibody into each well. Incubate 30 minutes at room temperature.
Add 100 μl prepared H
R
P-
Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µl T
M
B One-
Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
Note
This Product is for Research Use Only, not for use in in vitro diagnostics, following guidance from the FDA for tests submitted to the FDA for Emergency Use Authorization and Notification on March 16th, 2020, and amended on May 4th, 2020. While this test has been submitted for FDA EUA Authorization, it currently has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status. Please consult the manual for specific details and limitations of this test.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 Plate Kit
Available: In stock
available

Delivery expected until 6/28/2024 

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