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CLIA Kit for Corticosterone (Cort)

CLIA Kit for Corticosterone (Cort)

Item no.	CCA540Ge-48T
Manufacturer	Cloud-Clone
Amount	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Metabolism Diabetes Glucose Homeostasis ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	IA
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 275pg/mL
Citations	Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Corticosrone
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Chemiluminescent immunoassay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	Serum, plasma, urine and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Corticosterone (Cort). No significant cross-reactivity or interference between Corticosterone (Cort) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticosterone (Cort) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticosterone (Cort) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 100uL Substrate Solution. Incubate 10 minutes at 37° C; 7. Read RLU value immediately.
Test principle	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Corticosterone (Cort). A competitive inhibition reaction is launched between biotin labeled Corticosterone (Cort) and unlabeled Corticosterone (Cort) (Standards or samples) with the pre-coated antibody specific to Corticosterone (Cort). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticosterone (Cort) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Corticosterone (Cort) level in the sample or standard.
Manufacturer - Research Area	Infection immunity; Endocrinology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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