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ELISA Kit for Hyaluronic Acid (HA)

ELISA Kit for Hyaluronic Acid (HA)

Item no.	CEA182Ge-96T
Manufacturer	Cloud-Clone
Amount	96 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Category	Oncology Food Safety Allergenes ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Colorectal Cancer Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 1.77ng/mL
Citations	Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal Outcome Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway* Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in Egypt Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCV A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin. The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis Selected elements of extracellular matrix of the skin in diabetes and insulin resistance Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitis Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort An exploratory study to investigate the association between age, physical activity, femoral trochlear cartilage thickness and biomarkers of tissue metabolism in?¡ Trimethylamine N‐oxide exacerbates acetaminophen‐induced liver injury by interfering with macrophage‐mediated liver regeneration Comparative studies on the effect of pig adipose-derived stem cells (pASCs) preconditioned with hypoxia or normoxia on skin wound healing in mice Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathway Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Hyaluronan,Hyaluronate
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Hyaluronic Acid (HA). No significant cross-reactivity or interference between Hyaluronic Acid (HA) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronic Acid (HA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronic Acid (HA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A polyclonal antibody specific to hyaluronic acid has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hyaluronic acid and unlabeled hyaluronic acid (Standards or samples) with the pre-coated antibody specific to hyaluronic acid. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hyaluronic acid in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hyaluronic acid in the sample.
Manufacturer - Research Area	Tumor immunity; Hepatology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.