Comparison

ELISA Kit for Luteinizing Hormone (LH)

Item no. CEA441Mu-24T
Manufacturer Cloud-Clone
Amount 24 T
Quantity options 10x96 T 24 T 48 T 5x96 T 96 T
Category
Type Elisa-Kit
Specific against Mouse (Murine, Mus musculus)
Sensitivity The minimum detectable dose of this kit is typically less than 145.2pg/mL
Citations Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes.
PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone
Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes
Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines
Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering
Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis
Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility
Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice
A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis
Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring
New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.
Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis
Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity
Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells
Vitamin D deficiency impairs testicular development and spermatogenesis in mice
Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.
High sensitivity of testicular function to titanium nanoparticles
The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice
Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy
Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring
Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population
Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons
Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice
A Pex13 knockout in germ cells induces a spermatogenic arrest
Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons
基因敲除损伤小鼠睾丸睾酮产生与生精功能
Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling
Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 …
Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice
Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic …
Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice
BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose
Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells
Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice
Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in …
Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion
Polyamine metabolism links gut microbiota and testicular dysfunction
Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice
METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice
Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias ICSH; Lutropin; Interstitial Cell Stimulating Hormone
Available
Specificity Mus musculus (Mouse)
Quantity options
Detection range
370.4-30, 000pg/mL
Organism species
Mus musculus (Mouse)
Sensitivity
The minimum detectable dose of this kit is typically less than 145.2pg/mL
Sample type
serum, plasma and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity

This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).
No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone (LH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample.
Research Area
Endocrinology; Reproductive science; Hormone metabolism;
Reference
Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes.; PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone; Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes; Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines; Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering; Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis; Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility; Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice; A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis; Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring; New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.; Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis; Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity; Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells; Vitamin D deficiency impairs testicular development and spermatogenesis in mice; Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.; High sensitivity of testicular function to titanium nanoparticles; The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice; Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy; Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring; Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population; Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons; Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice; A Pex13 knockout in germ cells induces a spermatogenic arrest; Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons; 基因敲除损伤小鼠睾丸睾酮产生与生精功能; Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 24 T
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close