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ELISA Kit for Insulin (INS)

ELISA Kit for Insulin (INS)

Item no.	CEA448Mu-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 2.65pg/mL
Citations	Impaired neural stem/progenitor cell proliferation in Streptozotocin-induced and spontaneous diabetic miceSubcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse modelSubcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β.Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese miceBaicalin against obesity and insulin resistance through activation of AKT/AS160/GLUT4 pathwayGinsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed miceRed paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese miceIrisin Regulates Heme Oxygenase-1/ Adiponectin Axis in Perivascular Adipose Tissue and Improves Endothelial Dysfunction in Diet-Induced Obese MiceActiviated Galanin receptor 2 attenuates insulin resistance in skeletal muscle of obese miceEffects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese MiceSynthesis and anti-diabetic activity of new N,N-dimethylphenylenediamine-derivatized nitrilotriacetic acid vanadyl complexes.Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal musclePeripheral insulin resistance in ILK-depleted mice by reduction of GLUT4 expression.Beneficial effect of baicalin on insulin sensitivity in adipocytes of diet-induced obese mice组织蛋白酶G 在1 型糖尿病小鼠CD+ 4T 淋巴细胞活化过程中的作用.Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in MiceTranscriptomic analysis reveals effects of fucoxanthin on intestinal glucose transportEffect of Baicalein on GLUT4 Translocation in Adipocytes of Diet-Induced Obese MiceSilencing of LncRNA Steroid Receptor RNA Activator attenuates polycystic ovary syndrome in MiceGeniposide and Gentiopicroside Suppress Hepatic Gluconeogenesis via Regulation of AKT-FOXO1 PathwayTreatment with celastrol protects against obesity through suppression of galanin-induced fat intake and activation of PGC-1α/GLUT4 axis-mediated glucose …Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposureEffects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 MiceHuperzine A ameliorates obesity-related cognitive performance impairments involving neuronal insulin signaling pathway in miceGlycerol Monolaurate‐Mediated Attenuation of Metabolic Syndrome is Associated with the Modulation of Gut Microbiota in High Fat diet‐fed MiceBaicalin ameliorates hepatic insulin resistance and gluconeogenic activity through inhibition of p38 MAPK/PGC-1α pathwayMicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression ModelmiR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42/β-Catenin SignalingDepolymerized RG-I enriched pectin from citrus segment membrane modulates gut microbiota, increases SCFAs production, promotes the growth of Bifidobacterium …Isoflurane aggravates peripheral and central insulin resistance in high-fat diet/streptozocin-induced type 2 diabetic miceTriphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal miceLiraglutide improved inflammation via mediating IL-23/Th-17 pathway in obese diabetic mice with psoriasiform skinComposite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db miceHyperbaric oxygen ameliorates insulin sensitivity by increasing GLUT4 expression in skeletal muscle and stimulating UCP1 in brown adipose tissue in T2DM …Integrin Linked Kinase (ILK) Downregulation as an Early Event During the Development of Metabolic Alterations in a Short-Term High Fat Diet Mice Model.Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose …AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axisThioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunctionAdiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via …Insulin promotes corneal nerve repair and wound healing in type 1 diabetic mice by enhancing Wnt/β-catenin signalingAplysin Retards Pancreatic Necrosis and Inflammatory Responses in NOD Mice by Stabilizing Intestinal Barriers and Regulating Gut Microbial CompositionHighly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced ObesityRegulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes MellitusFucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut …The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance micePhosphorylation of eIF2¦Á signaling pathway attenuates obesity-induced non-alcoholic fatty liver disease in an ER stress and autophagy-dependent mannerEmpagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in miceTime-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1¦Á expression and activityPhysiological and genetic convergence supports hypoxia resistance in high-altitude songbirdsN1?methylnicotinamide ameliorates insulin resistance in skeletal muscle of type 2 diabetic mice by activating the SIRT1/PGC?1¦Á signaling pathwayIdentification of curcumin as a potential ¦Á©\glucosidase and dipeptidyl©\peptidase 4 inhibitor: Molecular docking study, in vitro and in vivo biological evaluationConsumption of soybean or olive oil at recommended concentrations increased the intestinal microbiota diversity and insulin sensitivity and prevented fatty liver?¡TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic diseaseDAla2-GIP-GLU-PAL Protects Against Cognitive Deficits and Pathology in APP/PS1 Mice by Inhibiting Neuroinflammation and Up-regulating cAMP/PKA/CREB?¡Baicalin protects against insulin resistance and metabolic dysfunction through activation of GALR2/GLUT4 signalingLactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeabilityTime-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of miceSpexin ameliorates skeletal muscle insulin resistance through activation of GAL2 receptorATP6V1H deficiency impairs glucose tolerance by augmenting endoplasmic reticulum stress in high fat diet fed miceEnvironmental exposure to low-dose perfluorohexanesulfonate promotes obesity and non-alcoholic fatty liver disease in mice fed a high-fat dietHepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistanceTreatment with spexin mitigates diet-induced hepatic steatosis in vivo and in vitro through activation of galanin receptor 2Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cellsConstitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Insulin (INS). No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.