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ELISA Kit for Progesterone (PG)

ELISA Kit for Progesterone (PG)

Item no.	CEA459Ge-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Food Safety Hormones Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 0.47ng/mL
Citations	Prevention of preterm birth by progestational agents: what are the molecular mechanisms? The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy The biocompatibility studies of polymer dots on pregnant mice and fetuses Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment Prenatal exposure of female mice to perﬂuorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Guoqi Liu1, Juan Li2, 3, Peng Cui2, Susanne Lager5, Amanda Nancy Sferruzzi-Perri6, Wei Li1, Xiao-ke Wu1, Yanhua Han1, Mats Brännström7, Linus R Shao2 … Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and … Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS Improving reproductive efficiency through the supplementation of mustard oil, poly-herbal mixture and butyric acid during the periparturient period in Sahiwal … A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡ MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	P4,Pregn-4-Ene-3,20-Dione
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Progesterone (PG). No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progesterone (PG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Progesterone (PG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Progesterone (PG) and unlabeled Progesterone (PG) (Standards or samples) with the pre-coated antibody specific to Progesterone (PG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Progesterone (PG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Progesterone (PG) in the sample.
Manufacturer - Research Area	Endocrinology; Reproductive science; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.