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ELISA Kit for Cortisol (Cor)

ELISA Kit for Cortisol (Cor)

Item no.	CEA462Ge-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Category	Food Safety Allergenes Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 5.15ng/mL
Citations	Use of infrared thermography to assess the influence of high environmental temperature on rabbits Identification of sociodemographic and clinical factors associatedwith the levels of human b-defensin-1 and human b-defensin-2in the human milk of Han Chinese Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model Effect of high temperatures on breeding rabbit behaviour Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation … A study on certain blood biomarkers of pregnant Cholistani cows. The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot Study Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexia Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirus Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense Anti-stress activity of some plants extracts of the North Caucasus flora Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets Stress response after race and endurance training sessions and competitions in Arabian horses Associations of Chinese social face with cortisol level and glucocorticoid receptor gene Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1 …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Hydrocortisone
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma, urine and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Cortisol (Cor). No significant cross-reactivity or interference between Cortisol (Cor) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cortisol (Cor) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cortisol (Cor) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cortisol (Cor) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cortisol (Cor) and unlabeled Cortisol (Cor) (Standards or samples) with the pre-coated antibody specific to Cortisol (Cor). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cortisol (Cor) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cortisol (Cor) in the sample.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.