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ELISA Kit for Thyroid Stimulating Hormone (TSH)

ELISA Kit for Thyroid Stimulating Hormone (TSH)

Item no.	CEA463Mu-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Metabolism Cholesterol & Lipid Metabolism ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Thyroid Cancer Lymphatic System Cancer
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 2.5pg/mL
Citations	Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis. Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of … Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid mice Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 131 Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Thyrotropin
Available
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH). No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyroid Stimulating Hormone (TSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyroid Stimulating Hormone (TSH) and unlabeled Thyroid Stimulating Hormone (TSH) (Standards or samples) with the pre-coated antibody specific to Thyroid Stimulating Hormone (TSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample.
Manufacturer - Research Area	Endocrinology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.