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ELISA Kit for Endothelin 1 (EDN1)

ELISA Kit for Endothelin 1 (EDN1)

Item no.	CEA482Hu-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Metabolism Cholesterol & Lipid Metabolism ELISA Cardiovascular Research Hypertension ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Heart & Blood Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 2.71pg/mL
Citations	B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients Endothelin 1 and endothelial dysfunction inchildren with idiopathic nephrotic syndrome Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients. Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight … Whitening effects of cosmetic formulation in the vascular component of skin pigmentation APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ET1,PPET1,Preproendothelin-1,Big endothelin-1
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 1 (EDN1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) and unlabeled Endothelin 1 (EDN1) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample.
Manufacturer - Research Area	Endocrinology; Cardiovascular biology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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