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ELISA Kit for Prostaglandin E2 (PGE2)

ELISA Kit for Prostaglandin E2 (PGE2)

Item no.	CEA538Ge-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Neural Transcription Factors Aging & Neurological Disorders ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Skin Cancer Bladder Cancer Testicular & Prostate Cancer Gastric Cancer Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 8.67pg/mL
Citations	Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis Lipid mediators in plasma of autism spectrum disorders Interleulin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage-dominant Microenvironment in Lung Cancer Artesunate exerts an anti-immunosuppressive effect on cervical cancer by inhibiting PGE2 production and Foxp3 expression. Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. Celecoxib attenuates hepatic cirrhosis through inhibition of epithelial-to-mesenchymal transition of hepatocytes Valproic acid protects septic mice from renal injury by reducing the inflammatory response Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway. Somatostatin prevents lipopolysaccharide Celecoxib enhanced the cytotoxic effect of cisplatin in drug-resistant human gastric cancer cells by inhibition of cyclooxygenase-2 Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE9 and is crucial for liver transplant tolerance Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery Artemisinin inhibits the proliferation, migration, and inflammatory reaction induced by tumor necrosis factor-伪 in vascular smooth muscle cells through nuclear factor kappa B pathway Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer IL-23/Th17 axis is not influenced by TNF-blocking agents in ankylosing spondylitis patients Metformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis Inhibition of cyclooxygenase-2 alleviates liver cirrhosis via improvement of the dysfunctional Cavia (Guinea pig )t-liver axis in rats Metformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis. Dietary Arachidonic Acid Has a Time-Dependent Differential Impact on Adipogenesis Modulated via COX and LOX Pathways in Grass Carp Ctenopharyngodon idellus. Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid. Metformin inhibits castration-induced EMT in prostate cancer by repressing COX2/PGE2/STAT3 axis. Identification of Biomarkers of Impaired Sensory Profiles among Autistic Patients. A Zinc Morpholine Complex Prevents HCl/Ethanol-Induced Gastric Ulcers in a Rat Model. Downregulation of HOTAIR Expression Mediated Anti-Metastatic Effect of Artesunate on CervicalCancer by Inhibiting COX-2 Expression. Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways. Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264. 7 macrophages through the MAPK and NF-κB … Sea cucumber saponins liposomes ameliorate obesity-induced inflammation and insulin resistance in high-fat diet-fed mice Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage Activation of PGE2/EP2 and PGE2/EP4 signaling pathways positively regulate the level of PD‑1 in infiltrating CD8+ T cells in patients with lung cancer Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid Metformin Inhibits Prostate Cancer Progression by Targeting Tumor-Associated Inflammatory Infiltration Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling Steroidal alkaloid solanine A from Linn. exhibits anti-inflammatory activity in lipopolysaccharide/interferon γ-activated murine macrophages and … Polar extract of protects cartilage homeostasis: possible mechanism of action Impaired lipid metabolism markers to assess the risk of neuroinflammation in autism spectrum disorder Phytochemical evaluation and anti-hemorrhoidal activity of bark of Acacia ferruginea DC Loganin prevents BV‐2 microglia cells from Aβ‐induced inflammation via regulating TLR4/TRAF6/NF‐κB axis Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: Histomorphometrical, Immunohistochemical, Imunological and Microbiological Analyses The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion Seasonal variations in hormones and enzymes of seminal plasma and its relationship with semen quality in crossbred cattle bulls Resveratrol enhances the protective effects of JBP485 against indomethacin-induced rat intestinal damage in vivo and vitro through up-regulating … Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard & Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Gastroprotective activity of a novel Schiff base derived dibromo substituted compound against ethanol-induced acute gastric lesions in rats Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer Chlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK Pathway 1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway Increased expression of TRPV1 in patients with acute or chronic cough after lung cancer surgery Melatonin attenuates expression of cyclooxygenase-2 (COX-2) in activated microglia induced by lipopolysaccharide (LPS) Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation Antidepressant-like effects of dietary gardenia blue pigment derived from genipin and tyrosine Synergistic effect of sea cucumber saponin and EPA-enriched phospholipids on insulin resistance in high-fat diet-induced obese mice Polydatin ameliorates chemotherapy-induced cognitive impairment (chemobrain) by inhibiting oxidative stress, inflammatory response, and apoptosis in rats Management of Citrus sinensis peels for protection and treatment against gastric ulcer induced by ethanol in rats Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3 … The Efficacy of Processing Strategies on the Gastroprotective Potentiality of Chenopodium quinoa Seeds FADS1 promotes the progression of laryngeal squamous cell carcinoma through activating AKT/mTOR signaling Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide Echinacea polysaccharide attenuates lipopolysaccharide‑induced acute kidney injury via inhibiting inflammation, oxidative stress and the MAPK signaling pathway Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors Fenretinide regulates macrophage polarization to protect against experimental colitis induced by dextran sulfate sodium Bromelain reduced pro-inflammatory mediators as a common pathway that mediate antinociceptive and anti-anxiety effects in sciatic nerve ligated Wistar rats Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis Assessment of Galectin-1, Galectin-3, and PGE2 Levels in Patients with COVID-19 Effect of a Proprietary Herbal Product" Arthrocon" on some Synovial Fluid and Serum Biomarkers in Horses with Naturally Occurring Osteoarthritis Induction of apoptosis in activated RAW 264.7 cells and inhibition of pro-inflammatory mediators in rat air pouch by ethylacetate fraction of Ocimum gratissimum leaves Novel 1, 3, 4-Oxadiazole Derivatives of Pyrrolo [3, 4-d] Pyridazinone Exert Anti-Inflammatory Activity without Acute Gastrotoxicity in the Carrageenan-Induced … Anti‑inflammation treatment for protection of hepatocytes and amelioration of hepatic fibrosis in rats Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus The ameliorative effects of cinnamon oil against ethanol-induced gastric ulcer in rats by regulating oxidative stress and promoting angiogenesis Suppression of COX-2/PGE2 levels by carbazole-linked triazoles via modulating methylglyoxal-AGEs and glucose-AGEs–Induced ROS/NF-κB signaling in monocytes
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PG-E2
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Prostaglandin E2 (PGE2). No significant cross-reactivity or interference between Prostaglandin E2 (PGE2) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGE2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGE2 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGE2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGE2 in the sample.
Manufacturer - Research Area	Metabolic pathway; Infection immunity; Endocrinology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.