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ELISA Kit for Malondialdehyde (MDA)

ELISA Kit for Malondialdehyde (MDA)

Item no.	CEA597Ge-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Protein Aggregation, Clearance & Misfolding ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Testicular & Prostate Cancer Neuronal Cell Markers
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 8.84ng/mL
Citations	Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κBEffect of intermittent hepatic inflow occlusion with the Pringle maneuver during donor hepatectomy in adult living donor liver transplantation with right hemiliver grafts: a prospective, randomized controlled studyFormaldehyde Impairs Learning and Memory Involving the Disturbance of Hydrogen Sulfide Generation in the Hippocampus of RatsThe amelioration of AH by abstinence and the attenuation of oxidative stressHigh salt intake accelerated cardiac remodeling in spontaneously hypertensive rats: time window of left ventricular functional transition and its relation to salt-loading dosesEffect of caffeic acid on doxorubicin induced cardiotoxicity in ratsIodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Chronic thromboembolic pulmonary hypertension is not associated with iron overloadOxidative stress status in elite athletes engaged in different sport disciplinesRole of Aldehyde Dehydrogenase 2 in 1-Methy-4-Phenylpyridinium Ion-Induced Aldehyde Stress and Cytotoxicity in PC12 CellsIschemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats.Metformin alleviates high glucoseInfluence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic SyndromeBDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 CellsInfluence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome Mitochondrial respiratory dysfunctions of blood mononuclear cells link with cardiac disturbance in patients with early-stage heart failureObstructive sleep apnea is independently associated with inflammation and insulin resistance, but not with blood pressure,Puerarin Attenuates Anoxia/Reoxygenation Injury Through Enhancing Bcl-2 Associated Athanogene 3 Expression, a Modulator of Apoptosis and AutophagyMechanism (s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calaburaEvaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanismHydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2Comparative Analysis of Some Antioxidant Markers in Periodontal DiseaseInfluence of Supplementing Diet with Microalgae (Schizochytrium limacinum) on Growth and Metabolism of Lambs during the Summer 2Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced CardiomyopathyPanax notoginseng saponins protect kidney from diabetes by up-regulating silent informationregulator 1 and activating antioxidant proteins in rats.Hydrogen sulfide inhibits MPP+-induced aldehyde stress and endoplasmic reticulum stress inPC12 cells: involving upregulation of BDNF.Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection.Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory CytokinesInhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide.Influence of supplementing diet with microalgae (Schizochytrium limacinum) on growth and metabolism in lambs during the summerHydrogen sulfide attenuates chronic restrain stress-induced cognitive impairment by upreglulation of Sirt1 in hippocampusResveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 1Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorderSex differences in complex regional pain syndrome type I (CRPS-I) in miceDyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stressBurn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress.Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oilEpigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayGold Nanoparticle Toxicity in Mice and Rats: Species DifferencesProtective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusionHydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsEpigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathwayHydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of …Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snailsEffects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayLead-induced increase in erythrocyte osmotic fragility and malondialdehyde concentration in Wistar rats: Chemo-protective effects of flavonoid mixtureEnoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosisAre thalassemia patients oxidatively challenged?Serum Uric Acid Levels are Associated with Cardiometabolic Risk Factors in Healthy Young and Middle-Aged AdultsHydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituentsImproved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromeProbucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsEffects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsProtective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsNaomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling PathwayExamination of Oxidative Stress Markers in Women with Postmenopausal OsteoporosisThe Relationship Between Serum Level of Malondialdehyde and Progression of Colorectal CancerThe effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injuryEuropean Journal of Biomedical AND Pharmaceutical sciencesThe cardiovascular phenotype of adult patients with phenylketonuriaHydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic FactorInfluence of deficient nutrition on trace element status and antioxidant defense systemGinsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injuryPhenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese RatsHydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the HippocampusNRF2 deficiency increases obesity susceptibility in a mouse menopausal modelSelected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location—A Preliminary ResearchExosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han populationRapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayImprovement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based ProteomicsEffects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler …year=2019volume=10issue=3spage=70epage=75aulast=Kadhim">Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional StudyTualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed ratsEffects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat OffspringThe Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular CarcinomaFormaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effectTualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed RatsEndostatin attenuates heart failure via inhibiting reactive oxygen species in myocardialDental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitisProtective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in ratsTanshinone IIA attenuates heart failure via inhibiting oxidative stress in myocardial infarction ratsAlarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure ratsNaringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic ratsMicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinomaκ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB PathwayAlamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl ratsPeripheral blood oxidative stress markers for obstructive sleep apnea—a meta-analysisIntake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseaseFibroblast growth factor 12 attenuated cardiac remodeling via suppressing oxidative stressTargeting of midkine alleviates cardiac hypertrophy via attenuation of oxidative stress and autophagyAntioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MiceThe κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in RatsMalondialdehyde and Neutrophil Gelatinase-Associated Lipocalin as Markers of Oxidative Stress in Small for Gestational Age Newborns from Hypertensive …Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic NeuropathyTransplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Malondialdehyde (MDA). No significant cross-reactivity or interference between Malondialdehyde (MDA) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Malondialdehyde (MDA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Malondialdehyde (MDA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Malondialdehyde (MDA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Malondialdehyde (MDA) and unlabeled Malondialdehyde (MDA) (Standards or samples) with the pre-coated antibody specific to Malondialdehyde (MDA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Malondialdehyde (MDA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Malondialdehyde (MDA) in the sample.
Manufacturer - Research Area	Metabolic pathway; Hepatology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.