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ELISA Kit for Folic Acid (FA)

ELISA Kit for Folic Acid (FA)

Item no.	CEA610Ge-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Food Safety Allergenes Vitamines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 285.77pg/mL
Citations	Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.Development of Orally Applicable, Combinatorial Drug–Loaded Nanoparticles for the Treatment of FibrosarcomaRole of folic acid deficiency as a possible risk factor for erectile dysfunctionLow serum folic acid can be a potential independent risk factor for erectile dysfunction: a prospective case–control studyEnhanced cellular uptake, transport and oral bioavailability of optimized folic acid-loaded chitosan nanoparticlesFolate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, Ethiopia
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	VB9,Vitamin B9,Folacin,Folate,V,Itamin M,Vitamin M,Vitamin Bc,Pteroyl-L-Glutamic Acid,Pteroyl-L-Glutamate
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Folic Acid (FA). No significant cross-reactivity or interference between Folic Acid (FA) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folic Acid (FA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folic Acid (FA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Folic Acid (FA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Folic Acid (FA) and unlabeled Folic Acid (FA) (Standards or samples) with the pre-coated antibody specific to Folic Acid (FA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Folic Acid (FA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Folic Acid (FA) in the sample.
Manufacturer - Research Area	Hematology; Reproductive science; Nutrition metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.